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Catalog #
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Source:
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Lot #
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Size:
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AP2726a
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Rabbit
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SH080523S
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_000025, P04075
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RB14944
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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39420 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ALDOA.
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Other Names:
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Fructose-bisphosphate aldolase A; EC 4.1.2.13; Muscle-type aldolase; Lung cancer antigen NY-LU-1; ALDOA; ALDA
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Application Data:
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Western blot analysis of anti-ALDOA Antibody (N-term) (Cat.#AP2726a) in CEM cell line lysates (35ug/lane). ALDOA(arrow) was detected using the purified Pab (1:60 dilution). |
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDOA antibody (N-term) (Cat.#AP2726a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.
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Background References:
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- Gizak,A., Proteins 72 (1), 209-216 (2008)
- Lu,J., Biochem. Biophys. Res. Commun. 369 (3), 948-952 (2008)
- Valis,K., Mol. Cell. Biochem. 311 (1-2), 225-231 (2008)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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