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S adenosylhomocysteine hydrolase (ACHY) Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP2733b Rabbit SH080527J 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_000678, P23526 RB14982 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H 47716 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human AHCY.
Other Names:
3D-structure; Acetylation; Cytoplasm; Direct protein sequencing; Hydrolase; NAD; One-carbon metabolism; Polymorphism
Application Data:
S adenosylhomocysteine hydrolase (ACHY) Antibody (C-term)

Western blot analysis of anti-AHCY Antibody (C-term)(Cat.#AP2733b) in HepG2 cell line lysates (35ug/lane). AHCY(arrow) was detected using the purified Pab (1:60 dilution).

 S adenosylhomocysteine hydrolase (ACHY) Antibody (C-term)

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AHCY antibody (C-term) (Cat.#AP2733b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Background:
S-adenosylhomocysteine hydrolase (AHCY) catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family.
Background References:
  1. Yideng,J.,DNA Cell Biol. 26 (8), 603-611 (2007)
  2. Arredondo-Vega,F.X.,Ann. Hum. Genet. 53 (PT 2), 157-167 (1989)
  3. Li,Q.S.,Biochemistry 47 (17), 4983-4991 (2008)
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:50~100
Immunohistochemistry1:10~50
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.