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Catalog #
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Source:
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Lot #
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Size:
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AP2746d
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Rabbit
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SH030404I
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_004307, P50553
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RB02897
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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25454 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding L68 of human Mash1.
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Other Names:
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Achaete-scute homolog 1; HASH1; ASCL1
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Application Data:
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Western blot analysis of Mash1 (arrow) using rabbit polyclonal Mash1 Antibody (L68) (Cat.#AP2746d).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Mash1 gene (Lane 2) (Origene Technologies). |
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Background:
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Mash1 is a member of the basic helix-loop-helix (BHLH) family of transcription factors. This protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in the gene encoding Mash1 may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.
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Background References:
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- Osada,H., Cancer Res. 68 (6), 1647-1655 (2008)
- Gregory,G.D., Mol. Cell. Biol. 27 (24), 8466-8479 (2007)
- Wang,X.Y., Lab. Invest. 87 (6), 527-539 (2007)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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