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Catalog #
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Source:
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Lot #
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Size:
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AP2785a
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Rabbit
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SH081103K
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001915, P24522
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RB16520
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H, M
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18205 Da predicted from amino acid sequence. Theoretical pI: 4.36
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human GADD45A.
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Other Names:
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Growth arrest and DNA-damage-inducible protein GADD45 alpha; DNA-damage-inducible transcript 1; DDIT-1; GADD45A; DDIT1; GADD45
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Application Data:
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Western blot analysis of anti-GADD45A Antibody (N-term) (Cat.#AP2785a) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab. |
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Background:
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GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45A gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.
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Background References:
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- Li,L.S., J. Biol. Chem. 283 (31), 21394-21403 (2008)
- Al-Romaih,K., Neoplasia 10 (5), 471-480 (2008)
- Zhu,Q.S., Cancer Res. 68 (8), 2895-2903 (2008)
- Kearsey,J.M., Oncogene 11 (9), 1675-1683 (1995)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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