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Catalog #
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Source:
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Lot #
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Size:
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AP3014a
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Rabbit
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SA060403A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_005148, P00519
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RB7351
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, DB, E
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H
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122873 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y251 of human ABL1.
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Other Names:
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Proto-oncogene tyrosine-protein kinase ABL1; p150; c-ABL; Abelson murine leukemia viral oncogene homolog 1; ABL1; ABL; JTK7;
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Application Data:
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(LEFT)Western blot analysis of anti-ABL Pab (Cat. #AP3014a) in Hela cell line lysate (35ug/lane). ABL(arrow) was detected using the purified Pab (1:60 dilution).
(RIGHT)Dot blot analysis of anti-Phospho-ABL1-Y251 Antibody (Cat. #AP3014a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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ABL1 is a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.
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Background References:
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- Barnes, K., et al., Oncogene 24(20):3257-3267 (2005).
- Kim, Y.R., et al., Genes Chromosomes Cancer 43(1):37-44 (2005).
- Aichberger, K.J., et al., Blood 105(8):3303-3311 (2005).
- Wei, G., et al., J. Biol. Chem. 280(13):12271-12278 (2005).
- Robinson, H.M., et al., Leukemia 19(4):564-571 (2005).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
| Dot Blot | 1:500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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