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Catalog #
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Source:
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Lot #
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Size:
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AP3094a
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Rabbit
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SA071129AH
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_004439, P04626
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RB12908
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, DB, IHC, E
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H
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137910 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1248 of human ErbB2.
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Other Names:
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Receptor tyrosine-protein kinase erbB-2 [Precursor]; p185erbB2; C-erbB-2; NEU proto-oncogene; Tyrosine kinase-type cell surface receptor HER2; MLN 19; ERBB2; HER2; NEU; NGL;
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Application Data:
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(LEFT)Phospho-ErbB2-Y1248 Antibody (cat# AP3074a)was used to detect Phospho-ErbB2-Y1248 in FG pancreatic carcinoma cell line lysate.
FG pancreatic carcinoma cells treated with or without EGF (50ng/ml) for 15 min
Phospho-ErbB2-Y1248 Antibody (cat# AP3074a) was used at 1:750 in 3% BSA.
Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
(RIGHT)Dot blot analysis of anti-Phospho-ErbB2-pY1248(M) Antibody (Cat.#AP3094b) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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ErbB2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors.
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Background References:
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- Provinciali, M., et al., Int. J. Cancer 115(1):36-45 (2005).
- Yee, L.D., et al., J. Nutr. 135(5):983-988 (2005).
- Beckers, J., et al., Int. J. Cancer 114(4):590-597 (2005).
- Brandt, B., et al., Biochem. Biophys. Res. Commun. 329(1):318-323 (2005).
- Ghatak, S., et al., J. Biol. Chem. 280(10):8875-8883 (2005).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Dot Blot | 1:500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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