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Catalog #
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Source:
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Lot #
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Size:
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AP3127a
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Rabbit
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SA060322H
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001547, O14920
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RB6996
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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86564 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y199 of human IKKb.
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Other Names:
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Inhibitor of nuclear factor kappa B kinase beta subunit; I-kappa-B-kinase beta; IkBKB; IKK-beta; IKK-B; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB; IKBKB; IKKB;
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Application Data:
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The anti-Phospho-IKKb-Y199 Pab (Cat. #AP3127a) is used in Western blot to detect Phospho-IKKb-Y199 in Y79 tissue lysate |
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Background:
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NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the inhibitory I-kappa-B proteins by kinases (IKKa or IKKb) leads to the dissociation of the inhibitor/NF-kappa-B complex and marks the inhibitors for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs.
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Background References:
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- Tomasoni, S., et al., Transplantation 79(9):1056-1061 (2005).
- Lappas, M., et al., Endocrinology 146(3):1491-1497 (2005).
- Carcamo, J.M., et al., Mol. Cell. Biol. 24(15):6645-6652 (2004).
- May, M.J., et al., J. Biol. Chem. 279(44):45528-45539 (2004).
- Ea, C.K., et al., Proc. Natl. Acad. Sci. U.S.A. 101(43):15318-15323 (2004).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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