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Catalog #
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Source:
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Lot #
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Size:
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AP3248a
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Rabbit
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SA060324I
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_005893, P84022
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RB7297
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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DB, E
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H
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48081 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S204 of human SMAD3.
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Other Names:
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Mothers against decapentaplegic homolog 3; SMAD 3; Mothers against DPP homolog 3; Mad3; hMAD-3; JV15-2; hSMAD3; SMAD3; MADH3;
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Application Data:
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Dot blot analysis of anti-Phospho-SMAD3-S204 Antibody (Cat. #AP3248a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
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Background:
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SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80% of
all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their C-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocates into the nucleus, where it regulates transcription of target genes. SMAD3 signal transduction appears to be important in the regulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.
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Background References:
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- Imoto, S., et al., FEBS Lett. 579(13):2853-2862 (2005).
- Dubrovska, A., et al., Oncogene 24(14):2289-2297 (2005).
- Furumatsu, T., et al., J. Biol. Chem. 280(9):8343-8350 (2005).
- Kobayashi, T., et al., Biochem. Biophys. Res. Commun. 327(2):393-398 (2005).
- Kamaraju, A.K., et al., J. Biol. Chem. 280(2):1024-1036 (2005).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Dot Blot | 1:500 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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