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Catalog #
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Source:
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Lot #
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Size:
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AP3254a
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Rabbit
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SA050831B
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_005424, P07947, P12931
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RB5973
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, DB, E
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H
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60670 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y537 of human YES1.
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Other Names:
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Proto-oncogene tyrosine-protein kinase Yes; p61-Yes; c-Yes; YES1; YES; Proto-oncogene tyrosine-protein kinase Src; EC 2.7.10.2; pp60c-src; p60-Src; c-Src
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Application Data:
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(TOP)Dot blot analysis of anti-Phospho-YES1-Y537/Phospho-SRC-Y530 Antibody (Cat.#AP3254a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
(BUTTOM)Western blot analysis of Phospho-YES1 Antibody Cat. #AP3254a in Jurkat cells. 40 ug of lysate loaded, ECL detection; Lane 1, 2.5 ug/ml; Lane 2, 1 ug/ml; Lane 3 0.62 ug/ml. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma |
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Background:
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This protein has tyrosine kinase activity and belongs to the src family of proteins. The YES1 gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene.
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Background References:
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- Anneren, C., et al., J. Biol. Chem. 279(30):31590-31598 (2004).
- Reinehr, R., et al., J. Biol. Chem. 279(23):23977-23987 (2004).
- Leclerc, P., et al., Biol. Reprod. 67(1):301-307 (2002).
- Oh, H.S., et al., J. Biol. Chem. 277(39):36489-36498 (2002).
- Silverman, G.A., et al., Genomics 15(2):442-445 (1993).
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Product Citations:
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- Michel Varrin-Doyer, et al. J. Biol. Chem. 2009; 284:13265-13276. (Applications: WB)
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Dot Blot | 1:500 |
| Western Blot | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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