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Catalog #
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Source:
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Lot #
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Size:
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AP3314a
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Rabbit
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SA070720C
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_005243, P01100
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RB11180
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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DB, E
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H
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40695 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T232 of human FOS.
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Other Names:
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Proto-oncogene protein c-fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7; FOS; G0S7;
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Application Data:
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Dot blot analysis of Phospho-FOS-T232 polyclonal antibody (Cat# AP3314a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide. |
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Background:
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The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
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Background References:
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- Gensch, E., et al., J. Biol. Chem. 279(37):39085-39093 (2004).
- Myllykangas, S., et al., Genes Chromosomes Cancer 40(4):334-341 (2004).
- Wu, M.Y., et al., World J. Gastroenterol. 10(4):476-480 (2004).
- Monje, P., et al., Mol. Cell. Biol. 23(19):7030-7043 (2003).
- Lavezzi, A.M., et al., Pathol. Int. 53(11):769-774 (2003).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Dot Blot | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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