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Catalog #
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Source:
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Lot #
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Size:
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AP3400a
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Rabbit
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SA071120AA
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_005914, Q99683
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RB12710
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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DB, E
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H
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154407 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S351 of human NR4A1.
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Other Names:
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MAPK/ERK kinase kinase 5, MEK kinase 5, MEKK 5, Apoptosis signal-regulating kinase 1, ASK-1, MAP3K5, ASK1, MAPKKK5, MEKK5
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Application Data:
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Dot blot analysis of Phospho-MAP3K5-S83 Antibody(Cat.#AP3400a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
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Background:
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Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
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Background References:
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- Dasgupta, P., et al., J. Biol. Chem. 279(37):38762-38769 (2004).
- Huang, S., et al., J. Biol. Chem. 279(35):36490-36496 (2004).
- Yasinska, I.M., et al., Arch. Biochem. Biophys. 428(2):198-203 (2004).
- Goldman, E.H., et al., J. Biol. Chem. 279(11):10442-10449 (2004).
- Park, H.S., et al., J. Biol. Chem. 279(9):7584-7590 (2004).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Dot Blot | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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