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Catalog #
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Source:
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Lot #
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Size:
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AP3495a
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Rabbit
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SA080303AT; SA081219AH
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_065812, Q8N122
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RB13350,RB16569
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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DB, E
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H
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149038 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S863 of human Raptor.
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Other Names:
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Regulatory-associated protein of mTOR; Raptor; P150 target of rapamycin (TOR)-scaffold protein; KIAA1303
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Application Data:
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Dot blot analysis of anti-raptor-pS863 Pab (RB13350) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
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Background:
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Raptor participates in the FRAP1 pathway and associates in a near stoichiometric ratio with FRAP1 to form a nutrient-sensitive complex (NSC). It plays a pivotal role as a scaffold protein in the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of EIF4EBP1. It has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1. Under nutrient-deprived conditions, raptor serves as a negative regulator of FRAP1 kinase activity. Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the rate of cell growth and maintenance of cell size with different environmental conditions.
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Background References:
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- Wang,Y., Circ. Res. 101 (6), 560-569 (2007)
- Fuchs,B.C., Am. J. Physiol., Cell Physiol. 293 (1), C55-C63 (2007)
- Olsen,J.V., Cell 127 (3), 635-648 (2006)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Dot Blot | 1:500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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