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Catalog #
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Source:
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Lot #
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Size:
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AP3537a
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Rabbit
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SA080527AR
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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Q13547
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RB8101
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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DB, E
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H
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55075 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S423 of human HDAC1.
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Other Names:
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Histone deacetylase 1; HD1; RPD3L1
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Application Data:
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Dot blot analysis of anti-Phospho-HDAC1-S423 Antibody (Cat.#AP3537a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
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Background:
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Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
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Background References:
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- Di Padova, M., et al., J. Biol. Chem. 278(38):36496-36504 (2003).
- Wang, S., et al., Oncogene 22(40):6204-6213 (2003).
- Xia, Z.B., et al., Proc. Natl. Acad. Sci. U.S.A. 100(14):8342-8347 (2003).
- Rocha, S., et al., Mol. Cell. Biol. 23(13):4713-4727 (2003).
- Macaluso, M., et al., Oncogene 22(23):3511-3517 (2003).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Dot Blot | 1:500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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