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Catalog #
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Source:
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Lot #
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Size:
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AP3550a
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Rabbit
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SA080911AO
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_055807, Q9Y4D1
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RB15348
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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DB, E
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H
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123473 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T361 of human DAAM1.
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Other Names:
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Disheveled-associated activator of morphogenesis 1;DAAM1;KIAA0666
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Application Data:
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Dot blot analysis of anti-Phospho-DAAM1-pT361 Antibody (Cat.#AP3550a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
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Background:
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Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton. Recent evidence suggests a role for the Formin homology (FH) proteins in these processes. DAAM1 contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by DAAM1, which is thought to function as a scaffolding protein.
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Background References:
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- Liu,W., Proc. Natl. Acad. Sci. U.S.A. 105 (1), 210-215 (2008)
- Yamashita,M.,Genes Cells 12 (11), 1255-1265 (2007)
- Lu,J., J. Mol. Biol. 369 (5), 1258-1269 (2007)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Dot Blot | 1:500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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