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Catalog #
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Source:
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Lot #
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Size:
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AP3558a
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Rabbit
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SA080911AL
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_006209, P42336
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RB15410
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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DB, E
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H
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124284 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y317 of human PK3CA.
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Other Names:
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Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; EC 2.7.1.153; PI3-kinase p110 subunit alpha; PtdIns-3-kinase p110; PI3K; PIK3CA
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Application Data:
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Dot blot analysis of anti-Phospho-PK3CA-pY317 Antibody (Cat.#AP3558a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
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Background:
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Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. This protein represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2.
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Background References:
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- Zhao,L., Oncogene 27 (41), 5486-5496 (2008)
- Volinia,S., Genomics 24 (3), 472-477 (1994)
- Hiles,I.D., Cell 70 (3), 419-429 (1992)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Dot Blot | 1:500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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