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ACE2 (SARS Receptor) Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP6020f Rabbit SH040108G 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_068576, Q9BYF1 RB4819 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H 92491 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ACE2.
Other Names:
Angiotensin-converting enzyme 2; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; ACEH; Processed angiotensin-converting enzyme 2; ACE2
Application Data:
ACE2 (SARS Receptor) Antibody (C-term)

(LEFT)The anti-ACE2 C-term Pab (Cat. #AP6020f) is used in Western blot to detect ACE2 in 293 cell lysate. (RIGHT)Western blot analysis of anti-ACE2 C-term Pab (Cat. #AP6020f) in K562 cell line lysates (35ug/lane). ACE2(arrow) was detected using the purified Pab.

 ACE2 (SARS Receptor) Antibody (C-term)

(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. (RIGHT)Formalin-fixed and paraffin-embedded human testis tissue reacted with ACE2 (SARS Receptor) Antibody (C-term) (Cat.#AP6020f), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Background:
ACE2 cDNA encodes a deduced 805-amino acid protein containing a potential 17-amino acid N-terminal signal peptide and a putative 22-amino acid C-terminal membrane anchor. It also possesses a zinc metalloprotease consensus sequence and a conserved glutamine residue that may function as a third zinc ligand. ACE2 is expressed predominantly in vascular endothelial cells of the heart and kidney. ACE converts angiotensin I to angiotensin II, ACE2 converts angiotensin I to angiotensin 1-9, which has 9 amino acids. Angiotensin II is a potent blood vessel constrictor, while angiotensin 1-9 does not impact blood vessels but is cleaved by ACE to a shorter peptide, angiotensin 1-7, which is a blood vessel dilator. Spike (S) proteins of coronaviruses, including the SARS coronavirus, bind with cellular receptors to mediate infection of target cells. ACE2 binds the S1 domain of the SARS coronavirus S protein. SARS coronavirus replicates efficiently on ACE2-transfected but not mock-transfected 293T cells. Anti-ACE2 but not anti-ACE1 antibody blocks viral replication on Vero E6 cells. It has been proposed that ACE2 is a functional receptor for SARS coronavirus.
Background References:
  1. Douglas, G.C., et al., Endocrinology 145(10):4703-4711 (2004).
  2. Turner, A.J., et al., Trends Pharmacol. Sci. 25(6):291-294 (2004).
  3. Towler, P., et al., J. Biol. Chem. 279(17):17996-18007 (2004).
  4. Wong, S.K., et al., J. Biol. Chem. 279(5):3197-3201 (2004).
  5. Li, W., et al., Nature 426(6965):450-454 (2003).
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.