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Catalog #
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Source:
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Lot #
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Size:
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AP6112a
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Rabbit
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SH030123A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_061337, P21439
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RB2209
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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140682 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the central region of human ABCB4.
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Other Names:
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Multidrug resistance protein 3, P-glycoprotein 3
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Application Data:
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Western blot analysis of anti-ABCB4 Ctr Pab (Cat. #AP6112a) in Mouse Heart tissue lysates. ABCB4 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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The membrane-associated protein encoded ABCB4 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function.
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Background References:
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- Eloranta, M.L., et al., Eur J Obstet Gynecol Reprod Biol 105(2):132-135 (2002).
- Eloranta, M.L., et al., Eur J Obstet Gynecol Reprod Biol 104(2):109-112 (2002).
- Jacquemin, E., Semin. Liver Dis. 21(4):551-562 (2001).
- Smit, J.J., et al., Biochim. Biophys. Acta 1261(1):44-56 (1995).
- Ruetz, S., et al., Cell 77(7):1071-1081 (1994).
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Product Citations:
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- Gautier, Thomas, et al. J. Lipid Res. 2007 Jan 01;48(1):30-40. (Applications: WB)
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Application Notes:
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| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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