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Catalog #
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Source:
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Lot #
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Size:
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AP6125a
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Rabbit
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SH021217G
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001158, P98170
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RB1793
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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56684 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human BIRC4.
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Other Names:
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Baculoviral IAP repeat-containing protein 4; Inhibitor of apoptosis protein 3; X-linked inhibitor of apoptosis protein; X-linked IAP; IAP-like protein; HILP; API3; IAP3; XIAP
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Application Data:
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(LEFT)The anti-BIRC4 N-term Pab (Cat. #AP6125a) is used in Western blot to detect BIRC4 in mouse liver tissue lysate.
(RIGHT)Western blot analysis of anti-BIRC4 Antibody (N-term) (Cat.#AP6125a) in Ramos cell line lysates (35ug/lane). BIRC4(arrow) was detected using the purified Pab. |
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with BIRC4 antibody (N-term) (Cat.#AP6125a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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BIRC4 is a member of a family of proteins which inhibit apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. Similar to API1, BIRC4 inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. BIRC4 also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7.
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Background References:
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- Srinivasula, S.M., et al., J. Biol. Chem. 278(34):31469-31472 (2003).
- Miranda, M.B., et al., Leukemia 17(2):390-400 (2003).
- Ahn, S.K., et al., Int. J. Mol. Med. 12(2):231-236 (2003).
- Yu, L.Y., et al., Mol. Cell. Neurosci. 22(3):308-318 (2003).
- Nomura, T., et al., Urol. Res. 31(1):37-44 (2003).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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