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Catalog #
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Source:
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Lot #
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Size:
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AP6140a
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Rabbit
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SH021219I
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_004711, Q9HBW0
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RB1827
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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39084 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human EDG4.
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Other Names:
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Lysophosphatidic acid receptor Edg-4; LPA receptor 2; LPA-2; LPA2
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Application Data:
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The anti-EDG4 N-term Antibody (Cat.#AP6140a) is used in Western blot to detect EDG4 in HL60 lysate. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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EDG4 is a member of family I of the G protein-coupled receptors, as well as the EDG family of proteins. This protein functions as a lysophosphatidic acid (LPA) receptor and contributes to Ca2+ mobilization, a critical cellular response to LPA in cells, through association with Gi and Gq proteins.
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Background References:
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- Hu, Y.L., et al., J. Natl. Cancer Inst. 95(10):733-740 (2003).
- Fujita, T., et al., Cancer Lett. 192(2):161-169 (2003).
- Contos, J.J., et al., Genomics 64(2):155-169 (2000).
- Zheng, Y., et al., FASEB J. 14(15):2387-2389 (2000).
- Young, K.W., et al., J. Biol. Chem. 275(49):38532-38539 (2000).
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Product Citations:
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- Horak, Christine E., et al. Cancer Res. 2007 Aug 01;67(15):7238-7246. (Applications: WB)
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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