|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AP6165a
|
Rabbit
|
SH030116N
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
NP_005353, P43357
|
RB2084
|
Rabbit Ig
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
WB, E
|
H
|
34747 Da
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MAGEA3.
|
|
Other Names:
|
|
Melanoma-associated antigen 3; MAGE-3 antigen; Antigen MZ2-D; MAGE3
|
|
Application Data:
|
The anti-MAGEA3 C-term Antibody (Cat.#AP6165a) is used in Western blot to detect MAGEA3 in Jurkat lysate. |
|
|
Background:
|
|
MAGEA3 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita.
|
|
Background References:
|
- Guo, J., et al., Exp. Mol. Pathol. 74(2):140-147 (2003).
- Consogno, G., et al., Blood 101(3):1038-1044 (2003).
- Rogner, U.C., et al., Genomics 29(3):725-731 (1995).
- Gaugler, B., et al., J. Exp. Med. 179(3):921-930 (1994).
- van der Bruggen, P., et al., Science 254(5038):1643-1647 (1991).
|
|
Product Citations:
|
- Kondo, Tetsuo, et al. Clin. Cancer Res. 2007 Aug 15;13(16):4713-4720. (Applications: WB)
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
