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Catalog #
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Source:
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Lot #
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Size:
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AP6265e
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Rabbit
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SA080306AC
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_000312, P06400
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RB7977
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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106159 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S608 of human RB1.
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Other Names:
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Retinoblastoma-associated protein; PP110; P105-RB; RB; RB1
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Application Data:
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Western blot analysis of anti-RB Antibody (S608) (Cat.#AP6265e) in Ramos cell line lysates (35ug/lane). RB1(arrow) was detected using the purified Pab. |
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Background:
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The retinoblastoma protein is a tumor suppressor protein that is dysfunctional in many types of cancer.[1] One highly studied function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. pRb belongs to the pocket protein family, whose members have a pocket for the functional binding of other proteins.[2][3] Should an oncogenic protein, such as those produced by cells infected by high-risk types of human papillomaviruses, bind and inactivate pRb, this can lead to cancer. Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral.
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Background References:
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- Dasgupta, P., et al., Mol. Cell. Biol. 24(21):9527-9541 (2004).
- Cui, X., et al., Hum. Pathol. 35(10):1189-1195 (2004).
- Borah, S., et al., J. Virol. 78(19):10336-10347 (2004).
- Dasgupta, P., et al., J. Biol. Chem. 279(37):38762-38769 (2004).
- Lohmann, D.R., et al., J. Biol. Chem. 129(1):23-28 (2004).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western Blot | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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