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Catalog #
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Source:
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Lot #
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Size:
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AP6293a
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Rabbit
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SH070723F
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_003368, Q16528
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RB11911
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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21602 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human VEGF2.
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Other Names:
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Vascular endothelial growth factor B [Precursor]; VEGF-B; VEGF-related factor; VRF; VEGFB; VRF;
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Application Data:
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Western blot analysis of anti-VEGF2 Antibody (N-term) Pab (AP6293a) in HL60 cell line lysates. VEGF2 Antibody (N-term)(arrow) was detected using the purified Pab. |
Formalin-fixed and paraffin-embedded skeletal muscle tissue reacted with VEGF2 Antibody (N-term)(Cat.#AP6293a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF. There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186. VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted. VEGFB stimulates endothelial cell proliferation. VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2. Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas. It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis. The genes that encode VEGFB have been mapped to chromosome 11q13.
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Background References:
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- Trompezinski, S., et al., Exp. Dermatol. 13(2):98-105 (2004).
- Qi, J.H., et al., Nat. Med. 9(4):407-415 (2003).
- Joukov, V., et al., J. Cell. Physiol. 173(2):211-215 (1997).
- Olofsson, B., et al., J. Biol. Chem. 271(32):19310-19317 (1996).
- Olofsson, B., et al., Proc. Natl. Acad. Sci. U.S.A. 93(6):2576-2581 (1996).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:250~500 |
| Immunohistochemistry | 1:10~50 |
| Immunoprecipitation | Not tested |
| Flow cytometry | Not tested |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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