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Catalog #
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Source:
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Lot #
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Size:
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AP6307b
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Rabbit
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SH040908B
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_004484, Q6IBS9
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RB6481
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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26923 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ABAD.
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Other Names:
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hydroxyacyl-Coenzyme A dehydrogenase, type II isoform 1; 3-hydroxyacyl-CoA dehydrogenase type II; Type II HADH; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; Endoplasmic reticulum-associated amyloid beta-peptide binding protein; Short-chain type dehydrogenase/reductase XH98G2 ; ERAB, HSD17B10, SCHAD; HADH2 protein
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Application Data:
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Western blot analysis of anti-ABAD Pab (Cat. #AP6307b) in mouse kidney tissue lysate.ABAD(arrow) was detected using the purified Pab. |
Immunohistochemistry on Formalin-fixed and paraffin-embedded human prostate tissue. (Top)Coloration with the Anti-ABAD antibody at a 1:20 concentration. (Bottom)Antibody adsorbed to the antigen (peptide), negative control.Disappearance of cytoplasmic staining indicating the antibody is specific. Data and protocol courtesy of Marie-Hélène Levesque, Centre de Recherche du CHUL, Canada. |
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Background:
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Amyloid b-peptide-binding alcohol dehydrogenase (ABAD) is a member of the family of short chain dehydrogenase/reductases; unique among this family, it binds amyloid b-peptide and exhibits enzymatic activity toward a wide variety of substrates including linear alcohols. In an amyloid beta-abundant environment, ABAD appears to trigger cell stress induced by the amyloid peptide.
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Background References:
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- FASEB J. 19 (6), 597-598 (2005)
- J. Mol. Biol. 342 (3), 943-952 (2004)
- Science 304 (5669), 448-452 (2004)
- FEBS Lett. 451 (3), 238-242 (1999)
- J. Biol. Chem. 274 (21), 15014-15019 (1999)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500" |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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