|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AP6321a
|
Rabbit
|
SH040717A
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/mL
|
NP_005542
|
RB6099
|
Rabbit Ig
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
WB, E
|
H
|
28671 Da
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminus of human KLK2.
|
|
Other Names:
|
|
Kallikrein 2, Tissue kallikrein 2, Glandular kallikrein-1, hGK-1
|
|
Application Data:
|
Western blot analysis of Kallikrein 2 (KLK2) antibody (N-term) (Cat.# AP6321a) in NCI-H460 cell line lysates (35ug/lane). Kallikrein 2 (arrow) was detected using the purified Pab. |
|
|
Background:
|
|
Kallikreins are a group of serine proteases manifesting diverse physiological functions. The glandular kallikreins are a distinct group of serine proteases with an ability to release vasoactive peptides from kininogen in vitro, although the kininogenase activity of different kallikreins is highly variable. Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Many kallikrein genes are differentially expressed in various malignancies. Human glandular kallikrein (hK2; encoded by the KLK2 gene) is an emerging tumor marker for prostate cancer.
|
|
Background References:
|
- Haese, A., et al., J Urol. 2005 Mar;173(3):752-6.
- Chiang, C.H., et al., J Urol. 2005 Feb;173(2):429-32.
- Clements, J.A., et al., Crit Rev Clin Lab Sci. 2004;41(3):265-312.
- Borgono, C.A., et al., Mol Cancer Res. 2004 May;2(5):257-80.
- Yousef, G.M., Clin Biochem. 2003 Sep;36(6):443-52.
|
|
Product Citations:
|
|
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western Blot | 1:50~100 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
