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Catalog #
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Source:
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Lot #
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Size:
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AP6347a
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Rabbit
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SH020613D
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_004498, O60921
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RB0455
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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31691 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a recombinant protein encoding full length of human HUS1.
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Other Names:
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Checkpoint protein HUS1; hHUS1; HUS1;
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Application Data:
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Western blot analysis of Hus1 (arrow) using rabbit polyclonal hHus1 (Cat. #AP6347a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Hus1 gene (Lane 2) (Origene Technologies). |
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Background:
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The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event.
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Background References:
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- Hirai, I., et al., Oncogene 23(30):5124-5130 (2004).
- Toueille, M., et al., (er) Nucleic Acids Res. 32(11):3316-3324 (2004).
- Scherer, S.W., et al., Science 300(5620):767-772 (2003).
- Kinzel, B., et al., Cancer 94(6):1808-1814 (2002).
- Roos-Mattjus, P., et al., J. Biol. Chem. 277(46):43809-43812 (2002).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blot | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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