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Catalog #
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Source:
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Lot #
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Size:
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AP6373a
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Rabbit
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SH080225I
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001609, P09874
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RB14153
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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112721 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the N-term of human PARP1.
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Other Names:
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Poly [ADP-ribose] polymerase 1; PARP-1; ADPRT; NAD(+) ADP-ribosyltransferase 1; Poly[ADP-ribose] synthetase 1; ADPRT; PPOL
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Application Data:
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Western blot analysis of PARP1 (arrow) using rabbit polyclonal PARP1 Antibody (N-term)(Cat.#AP6373a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PARP1 gene (Lane 2) (Origene Technologies). |
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Background:
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PARP1 is a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
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Background References:
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- Tao,Z., Biochemistry 47 (21), 5804-5813 (2008)
- Gao,R., Mol. Cancer Ther. 7 (5), 1246-1250 (2008)
- Schreiber,V., EMBO J. 11 (9), 3263-3269 (1992)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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