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Catalog #
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Source:
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Lot #
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Size:
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AP6555b
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Rabbit
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SH090710AE
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_000573, P10451
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RB18957
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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35423 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SPP1.
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Other Names:
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Osteopontin; Bone sialoprotein 1; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Nephropontin; Uropontin; OPN; BNSP; SPP1
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Application Data:
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Western blot analysis of SPP1 Antibody (C-term) (Cat. #AP6555b) in NCI-H460 cell line lysates (35ug/lane). SPP1 (arrow) was detected using the purified Pab. |
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Background:
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SPP1 binds tightly to hydroxyapatite. The protein appears to form an integral part of the mineralized matrix and probably important to cell-matrix interaction. It acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
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Background References:
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- Macri,A., Tumori 95 (1), 48-52 (2009)
- Agnihotri,R., J. Biol. Chem. 276 (30), 28261-28267 (2001)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at 20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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