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EEF1A1 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP6592a Rabbit SH090731BA 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_001393, P68104 RB19607 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, E H 50141 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human EEF1A1.
Other Names:
Elongation factor 1-alpha 1; EF-1-alpha-1; Elongation factor 1 A-1; eEF1A-1; Elongation factor Tu; EF-Tu; Leukocyte receptor cluster member 7; LENG7; EF1A; EEF1A; EEF1A1
Application Data:
EEF1A1 Antibody (N-term)

Western blot analysis of EEF1A1 antibody (N-term) (Cat. #AP6592a) in T47D cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab.

Background:
EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.
Background References:
  1. Byun,H.O., Cancer Res. 69 (11), 4638-4647 (2009)
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at 20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.