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Catalog #
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Source:
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Lot #
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Size:
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AP6682b
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Rabbit
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SH090902BB
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001062, P04818
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RB20041
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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35716 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human TYSY.
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Other Names:
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Thymidylate synthase; TSase; TS; TYMS
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Application Data:
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Western blot analysis of TYSY antibody (C-term) (Cat. #AP6682b) in Jurkat cell line lysates (35ug/lane). TYSY (arrow) was detected using the purified Pab. |
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Background:
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Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.
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Background References:
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- Ren,D.N., J Surg Oncol (2009)
- Schiffer,C.A., Biochemistry 34 (50), 16279-16287 (1995)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at 20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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