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Catalog #
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Source:
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Lot #
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Size:
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AP6693a
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Rabbit
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SH090907AT
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_006316, P62826
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RB20229
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H, M
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24423 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human RAN.
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Other Names:
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GTP-binding nuclear protein Ran; GTPase Ran; Ras-related nuclear protein; Ras-like protein TC4; Androgen receptor-associated protein 24; OK/SW-cl.81; ARA24; RAN
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Application Data:
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(LEFT) Western blot analysis of RAN Antibody (N-term) (Cat. #AP6693a) in HL-60 cell line lysates (35ug/lane).RAN (arrow) was detected using the purified Pab.
(RIGHT) Western blot analysis of RAN antibody (N-term) (Cat. #AP6693a) in NIH-3T3 cell line lysates (35ug/lane). RAN (arrow) was detected using the purified Pab. |
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Background:
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RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.
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Background References:
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- Monecke,T., Science 324 (5930), 1087-1091 (2009)
- Moss,D.K., J. Cell. Sci. 122 (PT 5), 644-655 (2009)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at 20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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