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Catalog #
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Source:
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Lot #
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Size:
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AP7005a
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Rabbit
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SH020812F
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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P35626
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RB0896
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H, M, Pr
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79677 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-term region of human GRK3.
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Other Names:
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G-protein coupled receptor kinase 3; Beta-adrenergic receptor kinase 2; Beta-ARK-2
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Application Data:
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The anti-GRK3 Pab (Cat. #AP7005a) is used in Western blot to detect GRK3 in SK-OV-3 cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2). |
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Background:
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The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the ADRBK2 enzyme, also known as GRK3, has 85% amino acid similarity with ADRBK1, with the protein kinase catalytic domain having 95% similarity. The ADRBK2 mRNA is approximately 8 kilobases with a distribution similar to that of ADRBK1. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.
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Background References:
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- Calabrese, G., et al., Genomics 23(1):286-288 (1994).
- Parruti, G., et al., Biochem. Biophys. Res. Commun. 190(2):475-481 (1993).
- Benovic, J.L., et al., J. Biol. Chem. 266(23):14939-14946 (1991).
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Flow cytometry | |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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