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Catalog #
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Source:
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Lot #
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Size:
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AP7063b
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Rabbit
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SH040514U
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_787062
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RB5431
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H, M
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46546 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human KIST.
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Other Names:
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Serine/threonine-protein kinase Kist; Kinase interacting with stathmin; KIS; U2AF homology motif kinase 1; UHMK1
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Application Data:
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The anti-KIST Pab (Cat. #AP7063b) is used in Western blot to detect KIST in K562 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2). |
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Background:
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KIST, a member of the Ser/Thr protein kinase family, is a pyruvate kinase that catalyzes formation of phosphoenolpyruvate from pyruvate and ATP. A role for the primarily nuclear KIST protein in mediation of cellular metabolism has been postulated based on the interaction identified with thyroid hormone. KIST is widely expressed, with highest abundance in skeletal muscle, kidney, placenta and peripheral blood leukocytes. Upon serum stimulation, KIST phosphorylates CDKN1B/p27Kip1, thereby regulating the subcellular location of CDKN1B and cell cycle progression in the G1 phase. KIST, which contains one RNA recognition motif domain, has been proposed to partipate in trafficking and processing of RNA. KIST binds to Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells.
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Background References:
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- Bieche, I., et al., Brain Res. Mol. Brain Res. 114(1):55-64 (2003).
- Boehm, M., et al., EMBO J. 21(13):3390-3401 (2002).
- Caldwell, B.D., et al., J. Biol. Chem. 274(49):34646-34656 (1999).
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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