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Catalog #
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Source:
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Lot #
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Size:
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AP7080a
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Rabbit
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SH040517P
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_060037
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RB5450
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H, M
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37375 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human NAGK.
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Other Names:
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N-acetylglucosamine kinase; GlcNAc kinase
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Application Data:
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The anti-NAGK Pab (Cat. #AP7080a) is used in Western blot to detect NAGK in mouse thymus tissue lysate (Lane 1) and Y79 cell lysate (Lane 2). |
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Background:
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N-acetylglucosamine kinase (NAGK) converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. NAGK belongs to the group of N-acetylhexosamine kinases and is a prominent salvage enzyme of amino sugar metabolism in mammals. The predicted 344-amino acid NAGK protein contains the 5 sequence motifs necessary for the binding of ATP by sugar kinases. NAGK shares 91.6% amino acid similarity with mouse Nagk, for which enzyme activity is detectable in all mouse tissues examined, with highest enzymatic activity in testis. It is hypothesized that NAGK has a general role in the catabolic pathways of GlcNAc as well as of ManNAc.
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Background References:
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- Hinderlich, S., et al., Eur. J. Biochem. 267(11):3301-3308 (2000).
- Lowes, W., et al., Biochim. Biophys. Acta 1379(1):134-142 (1998).
- Weidanz, J.A., et al., Br. J. Haematol. 95(4):645-653 (1996).
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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