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Catalog #
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Source:
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Lot #
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Size:
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AP7087a
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Rabbit
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SH040427I
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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AAV38745
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RB5274
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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30518 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human DCK.
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Other Names:
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Deoxycytidine kinase; dCK
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Application Data:
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Western blot analysis of hDCK-C9 (Cat. #AP7087a) in WiDr cell line lysates (35ug/lane). DCK (arrow) was detected using the purified Pab. |
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Background:
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Deoxycytidine kinase is responsible for the phosphorylation of several deoxyribonucleosides and their analogs. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. It is the rate limiting enzyme in the activation of many important anticancer and retroviral drugs and its activity is often decreased in cells that are resistant to cytosine arabinoside.
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Background References:
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- Chottiner, E. G., et al. Proc. Nat. Acad. Sci. 88: 1531-1535 (1991).
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Product Citations:
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blot | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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