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PKM2 Antibody (C-term C474)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP7173b Rabbit SH080624E 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_002645, P14618 RB15339 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H 57806 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKM2.
Other Names:
Pyruvate kinase, isozymes M1/M2; Pyruvate kinase muscle isozyme; Cytosolic thyroid hormone-binding protein; CTHBP; THBP1
Application Data:
PKM2 Antibody (C-term C474)

Western blot analysis of anti-PKM2 Antibody (C-term C474)(Cat.#AP7173b) in CEM cell line lysates (35ug/lane). PKM2(arrow) was detected using the purified Pab.

 PKM2 Antibody (C-term C474)

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PKM2 antibody (C-term C474) (Cat.#AP7173b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Background:
PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.
Background References:
  1. Lehner, B., et al., Genome Res. 14(7):1315-1323 (2004).
  2. Gevaert, K., et al., Nat. Biotechnol. 21(5):566-569 (2003).
  3. Valentini, G., et al., J. Biol. Chem. 277(26):23807-23814 (2002).
  4. Lowrie, D.J. Jr., et al., J. Struct. Biol. 132(2):83-94 (2000).
  5. Williams, J.M., et al., Mol. Microbiol. 27(1):171-186 (1998).
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:50~100
Immunohistochemistry1:10~50
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.