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Catalog #
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Source:
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Lot #
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Size:
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AP7176a
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Rabbit
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SH040521T
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_075394, O95544
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RB5607
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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49228 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PPNK.
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Other Names:
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Poly(P)/ATP NAD kinase; NAD kinase; NADK
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Application Data:
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Western blot analysis of NADK (arrow) using PPNK Antibody (N-term) (Cat.#AP7176a).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NADK gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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NADP is essential for biosynthetic pathways, energy, and signal transduction. Its synthesis is catalyzed by NAD kinase, which phosphorylates NAD+ to form NADP+. The NAD kinase gene is expressed in most human tissues, but not in skeletal muscle. The catalytically active homotetramer is highly selective for its substrates, NAD and ATP.
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Background References:
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- Lerner, F., et al., Biochem. Biophys. Res. Commun. 288(1):69-74 (2001).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western Blot | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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