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Catalog #
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Source:
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Lot #
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Size:
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AP7211c
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Rabbit
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SH081110H
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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Q16566
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RB15696
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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51925 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human CAMK4.
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Other Names:
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Calcium/calmodulin-dependent protein kinase type IV catalytic chain; CAM kinase-GR; CaMK IV
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Application Data:
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(Left)Western blot analysis of anti-CAMK4 Antibody (Center H353) Pab (Cat.#AP7211c) in HepG2 cell line lysates (35ug/lane). CAMK4 (arrow) was detected using the purified Pab.
(Right)Western blot analysis of CAMK4 (Center H353) (arrow) using rabbit polyclonal CAMK4 (Center H353) Antibody
(Cat.#AP7211c).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK4 (Center H353) gene (Lane 2)(Origene Technologies). |
Formalin-fixed and paraffin-embedded human brain tissue reacted with CAMK4 (Center H353), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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CAMK4 belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells.
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Background References:
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- Takai, N., et al., Cancer Lett. 183(2):185-193 (2002).
- Kang, H., et al., Cell 106(6):771-783 (2001).
- Wu, J.Y., et al., Nat. Genet. 25(4):448-452 (2000).
- Mosialos, G., et al., J. Virol. 68(3):1697-1705 (1994).
- Kitani, T., et al., J. Biochem. 115(4):637-640 (1994).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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