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Catalog #
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Source:
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Lot #
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Size:
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AP7218a
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Rabbit
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SH030516I
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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Q9UIK4
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RB3159
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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42898 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human DAPK2.
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Other Names:
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Death-associated protein kinase 2; DAP kinase 2; DAP-kinase related protein 1; DRP-1
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Application Data:
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(LEFT) The anti-DAPK2 Pab (Cat. #AP7218a) is used in Western blot to detect DAPK2 in mouse lung tissue lysate.
(RIGHT) Western blot analysis of DAPK2(arrow) using rabbit polyclonal DAPK2 Antibody (N-term V55) (Cat. #AP7218a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DAPK2 gene (Lane 2) (Origene Technologies). |
(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
(RIGHT)Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DAPK2 Antibody (N-term V55) (Cat.#AP7218a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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DAPK2 belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites.
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Background References:
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- Satoh, A., et al., Br. J. Cancer 86(11):1817-1823 (2002).
- Chan, M.W., et al., Clin. Cancer Res. 8(2):464-470 (2002).
- Wong, T.S., et al., Clin. Cancer Res. 8(2):433-437 (2002).
- Shani, G., et al., EMBO J. 20(5):1099-1113 (2001).
- Inbal, B., et al., Mol. Cell. Biol. 20(3):1044-1054 (2000).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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