|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AP7237c
|
Rabbit
|
SH30708G
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
NP_892010, Q9NYA1
|
RB3621
|
Rabbit Ig
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
WB, IHC, E, IP†
|
H, M, R, Pr
|
42517 Da
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the central region of human SPHK1.
|
|
Other Names:
|
|
Sphingosine kinase 1, SK 1, SPK 1
|
|
Application Data:
|
The anti-SPHK1 Pab (Cat. #AP7237c) is used in Western blot to detect SPHK1 in mouse kidney tissue lysate. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
|
|
Background:
|
|
Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.
|
|
Background References:
|
- Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
- Nava, V.E., et al., FEBS Lett. 473(1):81-84 (2000).
- Melendez, A.J., et al., Gene 251(1):19-26 (2000).
- Pitson, S.M., et al., Biochem. J. 350 Pt 2, 429-441 (2000).
|
|
Product Citations:
|
- Jun Li, et al. Clin. Cancer Res., Nov 2008; 14: 6996 - 7003. (Applications: WB)
- You, Bei, et al. Diabetes 2007 May 01;56(5):1445-1453. (Applications: WB)
- Sun, Jianxin, et al. Circ. Res. 2006 Sep 01;99(5):468-476. (Applications: IP)
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
† Application validated by third-party citation.
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
