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Catalog #
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Source:
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Lot #
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Size:
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AP7252a
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Rabbit
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SH020731U
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_006613, Q9NYY3
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RB0873
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M, R
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78236 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SNK.
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Other Names:
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Serine/threonine-protein kinase PLK2; Polo-like kinase 1; PLK-2; Serine/threonine-protein kinase SNK; Serum-inducible kinase; PLK2
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Application Data:
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The anti-SNK C-term Pab (Cat. #AP7252a) is used in Western blot to detect SNK in PMA-treated Pam212 cell lysate (lane 1) and rat testis tissue lysate (lane 2). |
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SNK Antibody (C-term ) (Cat.#AP7252a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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Plks (polo-like kinases) encode serine/threonine kinases that are closely
related to polo and CDC5, genes that are required for passage through mitosis
in Drosophila and Saccharomyces, respectively. Polo-like kinases, which
include Plk, Snk (for serum-inducible kinase, also designated Plk2) and Fnk
(for FGF-inducible kinase, also designated Plk3 or PRK), play a role in cell
proliferation. Plk protein accumulates in the cell during S and G2 phases of
the cell cycle, and both protein content and catalytic activity peak at the
onset of mitosis, followed by a rapid reduction after mitosis. Snk and Fnk are
immediate-early response genes that are first expressed during G1 phase. SNK may play a role in the division of at least some cell types, such as fibroblasts, and could function in embryogenesis, wound healing or neoplasia SNK mRNA is rapidly induced in human lung fibroblasts upon reintroduction of serum following 36 hours of serum deprivation.
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Background References:
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- Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
- Liby, K., et al., DNA Seq. 11:527-533 (2001).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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