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Catalog #
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Source:
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Lot #
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Size:
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AP7253b
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Rabbit
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SH021009I
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_065172
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RB1249
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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53128 Da
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Target/Specificity:
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Rabbits were immunized wth a KLH conjugated synthetic peptide selected from the C-terminal region of human CaMKIG.
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Other Names:
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calcium/calmodulin-dependent protein kinase IG
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Application Data:
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Western blot analysis of anti-CAMK 1G Pab (Cat. #AP7253b) in NCI-H460 cell lysate. CAMK 1G (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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Ca2+/calmodulin-dependent protein kinase I (CaMKI) constitutes a family of closely related isoforms (alpha, beta and gamma). CLICK-III/CaMKIgamma is a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase. AMKIgamma is abundant in neurons, particularly in the amygdala and ventromedial hypothalamus. Like the other CaMKI isoforms, full activation of CLICK-III/CaMKIgamma requires both Ca(2+)/CaM and phosphorylation by CaMKK.
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Background References:
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- Takemoto-Kimura, S., et al., J. Biol. Chem. 278(20):18597-18605 (2003).
- Schutte, B.C., et al., Genome Res. 10(1):81-94 (2000).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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