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Catalog #
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Source:
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Lot #
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Size:
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AP7254a
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Rabbit
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SH081110G
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001790, P50613
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RB15694
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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39038 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human CDK7.
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Other Names:
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Cell division protein kinase 7; CDK-activating kinase; CAK; TFIIH basal transcription factor complex kinase subunit; 39 kDa protein kinase; P39 Mo15; STK1; CAK1; CDK7; MO15
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Application Data:
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Western blot analysis of CDK7 (arrow) using rabbit polyclonal CDK7 Antibody (N-term K42) (Cat. #AP7254a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK7 gene (Lane 2). |
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Background:
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The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.
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Background References:
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- Zhou, M., et al., Proc. Natl. Acad. Sci. U.S.A. 100(22):12666-12671 (2003).
- Kino, T., et al., Biochem. Biophys. Res. Commun. 298(1):17-23 (2002).
- Schneider, E., et al., Oncogene 21(33):5031-5037 (2002).
- Nekhai, S., et al., Virology 266(2):246-256 (2000).
- Zhou, M., et al., Mol. Cell. Biol. 20(14):5077-5086 (2000).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at 20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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