|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AP7294c
|
Rabbit
|
SH080321B
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
NP_054699, Q08345
|
RB14442
|
Rabbit Ig
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
WB, E
|
H
|
101128 Da
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the Center of human DDR1.
|
|
Other Names:
|
|
Epithelial discoidin domain-containing receptor 1 [Precursor]; Epithelial discoidin domain receptor 1; Tyrosine kinase DDR; Discoidin receptor tyrosine kinase; Tyrosine-protein kinase CAK; Cell adhesion kinase; TRK E; Protein-tyrosine kinase RTK 6; HGK2; CD167 antigen-like family member A; CD167a antigen; DDR1
|
|
Application Data:
|
Western blot analysis of DDR1 (arrow) using rabbit polyclonal DDR1 Antibody (Center)(Cat.#AP7294c).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDR1 gene (Lane 2) (Origene Technologies). |
|
|
Background:
|
|
Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. DDR1 is an RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. It belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis show that expression of DDR1 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain.
|
|
Background References:
|
- Johnson J.D.,Proc. Natl. Acad. Sci. U.S.A. 90:5677-5681(1993)
- Sakuma S.,FEBS Lett. 398:165-169(1996)
|
|
Product Citations:
|
|
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50-100 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
