|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AP7415b
|
Rabbit
|
SH020626K
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
O00255, NP_000235
|
RB0543
|
Rabbit Ig
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
WB, IHC, E
|
H
|
67993 Da
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T594 of human MEN1.
|
|
Other Names:
|
|
Menin; MEN1; SCG2
|
|
Application Data:
|
Western blot analysis of anti-MEN1 Antibody (T594) (Cat.#AP7415b) in Ramos cell line lysates (35ug/lane). MEN1(arrow) was detected using the purified Pab. |
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MEN1 Antibody (T594) (Cat.#AP7415b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
|
|
Background:
|
|
Menin (MEN1) is a putative tumor suppressor associated with a syndrome known as multiple endocrine neoplasia type 1. In vitro studies have shown menin is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD, however, the function of this protein is not known.
|
|
Background References:
|
- Hashimoto,M., Int. J. Oncol. 33 (2), 333-340 (2008)
- Vidal,A., J Eur Acad Dermatol Venereol 22 (7), 835-838 (2008)
- Pieterman,C.R., Clin. Endocrinol. (Oxf) (2008)
|
|
Product Citations:
|
|
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western Blot | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
