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Catalog #
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Source:
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Lot #
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Size:
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AP7417b
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Rabbit
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SH080717G
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001211, Q13554
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RB15449
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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72727 Da predicted for full length protein. Multiple shorter isoforms known.
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CAMK2B.
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Other Names:
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Calcium/calmodulin-dependent protein kinase type II beta chain; CaM-kinase II beta chain; CaM kinase II subunit beta; CaMK-II subunit beta; EC 2.7.11.17; CAMK2B; CAM2; CAMKB
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Application Data:
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Western blot analysis of anti-CAMK2B Antibody (C-term D406) (Cat.#AP7417b) in CEM cell line lysates (35ug/lane). CAMK2B(arrow) was detected using the purified Pab. |
Formalin-fixed and paraffin-embedded human brain tissue reacted with CAMK2B (C-term D406) (Cat.#AP7417b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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CAMK2B belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. This protein is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin.
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Background References:
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- Hoogenraad,C.C., Dev. Cell 12 (4), 587-602 (2007)
- Novak,G., Synapse 59 (1), 61-68 (2006)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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