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Catalog #
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Source:
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Lot #
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Size:
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AP7438c
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Rabbit
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SA090409AB
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_620594, Q76LX8
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RB18029
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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153604 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human ADAMTS13.
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Other Names:
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von Willebrand factor-cleaving protease; vWF-cleaving protease; vWF-CP; A disintegrin and metalloproteinase with thrombospondin motifs 13; ADAMTS-13; ADAM-TS 13; ADAM-TS13; ADAMTS13; C9orf8
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Application Data:
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Western blot analysis of anti-ADAMTS13 Antibody (Center)(Cat.#AP7438c) in CEM cell line lysates (35ug/lane). ADAMTS13 (arrow) was detected using the purified Pab. |
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Background:
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ADAMTS13 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura.
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Background References:
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- Zheng X., Chung D., Takayama T.K.J. Biol. Chem. 276:41059-41063(2001)
- Levy G.G., Nichols W.C.Nature 413:488-494(2001)
- Cal S., Obaya A.J., Llamazares M., Garabaya C.Gene 283:49-62(2002)
- Zheng X., Nishio K., Majerus E.M.J. Biol. Chem. 278:30136-30141(2003)
- Anderson P.J., Kokame K., Sadler J.E.J. Biol. Chem. 281:850-857(2006)
- Pimanda J.E., Maekawa A., Wind T.Blood 103:627-629(2004)
- Plaimauer B., Fuhrmann J., Mohr G.Blood 107:118-125(2006)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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