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GPT Antibody (N-term R133)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP7468b Rabbit SH090903BF 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_005300, P24298 RB20083 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, E H 54637 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human GPT.
Other Names:
Alanine aminotransferase 1; ALT1; Glutamic--pyruvic transaminase 1; Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1 ; GPT1; AAT1; GPT
Application Data:
GPT Antibody (N-term R133)

Western blot analysis of GPT antibody (N-term R133) (Cat. #AP7468b) in K562 cell line lysates (35ug/lane). GPT (arrow) was detected using the purified Pab.

Background:
GPT(Glutamate-pyruvate transaminase), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. This protein has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2). See ALTQTL1 and ALTQTL2 for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.
Background References:
  1. Yang R.-Z., Blaileanu G.Genomics 79:445-450(2002)
  2. Ishiguro M., Takio K.Biochemistry 30:10451-10457(1991)
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at 20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.