primary antibodies and peptides 10239 Flanders Court, San Diego, CA 92121, USA. sales@abgent.com
Tel: 858.622.0099, 888-735-7227 (US toll free) Fax: 858-622-0609 (US toll free)

ALAS1 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP7486b Rabbit SH090430E 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_000679, P13196 RB17973 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, E H 70581 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ALAS1.
Other Names:
5-aminolevulinate synthase, nonspecific, mitochondrial; 5-aminolevulinic acid synthase; Delta-aminolevulinate synthase; Delta-ALA synthetase; ALAS-H; OK/SW-cl.121; ALASH; ALAS3
Application Data:
ALAS1 Antibody (C-term)

Western blot analysis of ALAS1 antibody (C-term) (Cat.#AP7486b) in Ramos cell line lysates (35ug/lane). ALAS1 (arrow) was detected using the purified Pab.

Background:
ALAS1 catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This protein is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2.
Background References:
  1. Zheng,J., Shan,Y. Mol. Cell. Biochem. 319 (1-2), 153-161 (2008)
  2. May,B.K., Bhasker,C.R. Mol. Biol. Med. 7 (5), 405-421 (1990)
  3. Tsang,H.T., Connell,J.W. Genomics 88 (3), 333-346 (2006)
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.