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Catalog #
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Source:
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Lot #
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Size:
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AP7486b
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Rabbit
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SH090430E
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_000679, P13196
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RB17973
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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70581 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ALAS1.
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Other Names:
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5-aminolevulinate synthase, nonspecific, mitochondrial; 5-aminolevulinic acid synthase; Delta-aminolevulinate synthase; Delta-ALA synthetase; ALAS-H; OK/SW-cl.121; ALASH; ALAS3
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Application Data:
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Western blot analysis of ALAS1 antibody (C-term) (Cat.#AP7486b) in Ramos cell line lysates (35ug/lane). ALAS1 (arrow) was detected using the purified Pab. |
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Background:
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ALAS1 catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This protein is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2.
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Background References:
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- Zheng,J., Shan,Y. Mol. Cell. Biochem. 319 (1-2), 153-161 (2008)
- May,B.K., Bhasker,C.R. Mol. Biol. Med. 7 (5), 405-421 (1990)
- Tsang,H.T., Connell,J.W. Genomics 88 (3), 333-346 (2006)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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