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Catalog #
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Source:
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Lot #
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Size:
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AP7500a
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Rabbit
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SH020827F
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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P27361
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RB0980
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M, R, Pr
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43135 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ERK1.
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Other Names:
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Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1, Microtubule-associated protein-2 kinase
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Application Data:
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(LEFT) The anti-ERK1 N-term Pab (Cat. #AP7500a) is used in Western blot to detect ERK1 in serum-starved HeLa cell lysate (lane 1) and rat skeletal muscle tissue lysate (lane 2).
(RIGHT) Western blot analysis of ERK1 (arrow) using rabbit ERK1 N-term Pab (Cat. #AP7500a).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK1 gene (Lane 2) (Origene Technologies). |
(TOP)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
(BUTTOM)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK1 antibody (N-term) (Cat.#AP7500a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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ERK1, a member of the MAP kinase subfamily of Ser/Thr protein kinases, is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. It phosphorylates EIF4EBP1 and is required for initiation of translation. The protein also phosphorylates microtubule-associated protein-2 (MAP2). ERK1 is activated and tyrosine phosphorylated in response to insulin and NGF.
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Background References:
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- Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
- Charest, D.L., et al., Mol. Cell. Biol. 13(8):4679-4690 (1993).
- Owaki, H., et al., Biochem. Biophys. Res. Commun. 182(3):1416-1422 (1992).
- Gonzalez, F.A., et al., FEBS Lett. 304 (2-3), 170-178 (1992).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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