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Catalog #
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Source:
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Lot #
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Size:
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AP7527b
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Rabbit
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SH030520F
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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P38936
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RB3180
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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18119 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CDKN1A.
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Other Names:
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Cyclin-dependent kinase inhibitor 1, p21, CDK-interacting protein 1, Melanoma differentiation associated protein 6, MDA-6
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Application Data:
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The anti-CDKN1A Pab (Cat. #AP7527b) is used in Western blot to detect CDKN1A in T-47D cell lysate. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Background:
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CDKN1A is a potent cyclin-dependent kinase inhibitor. It binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.
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Background References:
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- Fukuchi, K., et al., Biochim. Biophys. Acta 1642(3):163-171 (2003).
- Frouin, I., et al., J. Biol. Chem. 278(41):39265-39268 (2003).
- Dupont, J., et al., J. Biol. Chem. 278(39):37256-37264 (2003).
- Di Padova, M., et al., J. Biol. Chem. 278(38):36496-36504 (2003).
- Bai, Y.Q., et al., Oncogene 22(39):7942-7949 (2003).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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